Тип публикации: статья из журнала

Год издания: 2025

Идентификатор DOI: 10.1002/bio.70082

Аннотация: <jats:title>ABSTRACT</jats:title><jats:p>Bioluminescence inhibition (BLI) measurements in bioluminescent bacteria (BB) is perceived as a potential qualitative and quantitative indicator of hazardous materials. Acute but minor fluctuations in osmolarity and pH do not affect the living systems significantly. However, significant BLI Показать полностьюis observed from marine BB due to acute osmolarity or pH changes that may affect the bioassay sensitivity. Often, real samples have low pH and osmolarity, interfering with the hazard assessment based on the principles of BLI. This anomaly in BLI measurements may lead to false positives. Therefore, modifications in existing analytical methods to overcome such practical constraints are envisaged. In the present research, a marine BB was utilized to study the luminescence reversal effect when exposed to stressful environments such as hypotonic (deionized water), acidic (50 μM to 50 mM HCl), and 0.1–100 ppm of Hg(II) for 0–30 min. Postincubation, the calcium alginate immobilized bioluminescent bacteria (biophotonic beads) were transferred to Boss media to observe any luminescence enhancement. The results showed that osmotic shock and low-strength acidic environments (50 μM to 0.5 mM HCl) at specified incubation times were not detrimental to the biophonic beads regarding luminescence response.</jats:p>

Журнал: Luminescence

Выпуск журнала: Т. 40, № 1

ISSN журнала: 15227235

Издатель: John Wiley &amp; Sons, Incorporated

• Ranjan Rajeev (Department of Biomedical Science, Acharya Narendra Dev College University of Delhi New Delhi India)
• Goswami Sakshi (Toxicology laboratory, Department of Toxicology Chaudhary Charan Singh University Meerut India)
• Sharma Navendu (Toxicology laboratory, Department of Toxicology Chaudhary Charan Singh University Meerut India)
• Vashishtha Lalit Mohan (Toxicology laboratory, Department of Toxicology Chaudhary Charan Singh University Meerut India)
• Singh Meenu (Toxicology laboratory, Department of Toxicology Chaudhary Charan Singh University Meerut India)
• Verma Yeshvandra (Toxicology laboratory, Department of Toxicology Chaudhary Charan Singh University Meerut India)
• Rana Suresh Vir Singh (Toxicology laboratory, Department of Toxicology Chaudhary Charan Singh University Meerut India)
• Kratasyuk Valentina (Laboratory of Bioluminescent Biotechnologies, Department of Biophysics, Institute of Fundamental Biology and Biotechnology Siberian Federal University Krasnoyarsk Russia)
• Kumar Satyandra (Toxicology laboratory, Department of Toxicology Chaudhary Charan Singh University Meerut India)
• Pandey Archna (Department of Biomedical Science, Acharya Narendra Dev College University of Delhi New Delhi India)

• Ядро РИНЦ (eLIBRARY.RU)