Тип публикации: статья из журнала
Год издания: 2023
Идентификатор DOI: 10.1134/S0026893323050059
Ключевые слова: digital PCR, dPCR, infection, dna, oncology, virus, covid-19
Аннотация: Digital PCR (dPCR) is a nucleic acid quantification method that is widely used in genetic analysis.One of the most significant advantages of dPCR over other methods is the possibility of absolute quantitative determination of genetic material without construction of calibration curves, which allows one to detect even single moleculПоказать полностьюes of nucleic acids, and, hence, provides early diagnosis of diseases. One specific characteristicof dPCR is the detection of the analyzed biological object in each microreaction, followed by the presentationof the analysis results in a binary system, thereby giving the method its name. The key aspects of developingthe dPCR method, i.e., from the first devices based on microfluidic chip technology to modern systemscapable of measuring a target at a concentration of up to 1 in 100000 copies are shown in the current work.We analyzed the data on the detection of various pathogens using dPCR, as well as summarizing various studyresults demonstrating the innovativeness of this method. Both the possibilities of multiplex dPCR analysisand its potential in clinical practice are presented. This review also addresses the issue of the role of dPCR inthe development of noninvasive methods for analysis of oncological diseases. Possible ways of developingdPCR technology were emphasized, including its use as a “point-of-care” system.
Журнал: Molecular Biology
Выпуск журнала: Т. 57, № 5
Номера страниц: 793-801
ISSN журнала: 00268933
Место издания: Moscow
Издатель: Pleiades Publishing, Ltd.