Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций

Конференция: The 22nd International Charles Heidelberger Symposium on Cancer Research; Tomsk; Tomsk

Год издания: 2018

Ключевые слова: melanoma, non-cancerous melanoma tissue, microRNA

Аннотация: MicroRNA as epigenetic regulators has impact on melanoma development and progression. In this study, we aimed to identify the microRNA profile in melanoma and adjacent noncancerous tissue. Skin biopsies (n=21) obtained from melanoma patients were macrodissected. The reverse transcription reaction was performed in a reaction mixtureПоказать полностьюcontaining 0.1 μl of 5× primers miRNATaqMan assays specific to the investigated microRNAs and endogenous controls (Cat. № 4427975, Applied Biosystems). Expression levels of miRNAs were determined by real-time PCR using specific primers for miR-363-3p, miR-3591, miR-18a-5p, and miR-146a-5p (Cat. № 4427975,Applied Biosystems). Results. miR-146a-5p level was demonstrated as elevated in melanoma cells versus noncancerous adjacent tissue (p=0.002). 38 target genes have been determined for miR-146a-5p of which NRAS gene is known as one of the most frequent mutated in melanoma. Immunohistochemical evaluation of NRAS protein identified its increased level in NRAS-negative tumor tissue as compared to NRAS-mutant tumors (p=0.004). Elucidation of the role of miR-146-a-5p in complex interactions between the tumor and the surrounding cells is necessary for our understanding of the mechanisms of tumor progression and the development of new treatments.

Журнал: The 22nd International Charles Heidelberger Symposium on Cancer Research

Номера страниц: 6-7

Издатель: Национальный исследовательский Томский государственный университет

• Aksenenko M. B. (Federal State Budget Educational Institution of Higher Education «Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky» of the Ministry of Health of the Russian Federation)
• Palkina N. V. (Federal State Budget Educational Institution of Higher Education «Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky» of the Ministry of Health of the Russian Federation)
• Komina A. V. (Federal State Budget Educational Institution of Higher Education «Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky» of the Ministry of Health of the Russian Federation)
• Ruksha T. G. (Federal State Budget Educational Institution of Higher Education «Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky» of the Ministry of Health of the Russian Federation)

• РИНЦ (eLIBRARY.RU)