Тип публикации: статья из журнала
Год издания: 2016
Идентификатор DOI: 10.4028/www.scientific.net/KEM.683.506
Ключевые слова: surfactant, modified heptamethyltrisiloxane, Tween-20, GUS-assay, polymerase chain reaction, electrophoresis, cDNA, rna
Аннотация: Detergents represent a unique class of chemical compounds. They can alter surface and interphase bonds, and form micellar systems. These detergent properties allow to alter wettability of surfaces, stabilize or destabilize dispersed systems, and modify the properties of liquid phases. Therefore, the use of detergents is virtually uПоказать полностьюnlimited in chemical synthesis and processing, medicine, biological systems and agricultural biology. The article includes the studies of the feasibility of application of 1,1,1,3,5,5,5-heptamethyltrisiloxane modified by polyalkylene oxide in combination with allyloxypolyethyleneglycol in the ratio of 10:1 as a detergent for agrobacterialmediated transformation. Tween 20 detergent was used as a means of control in the concentration of 5%. As a result of histochemical analysis of transformed tissues, a significant difference was determined in expression of beta-glucuronidase reporter gene. The study of gene expression by calculating the relative content of mRNA showed that the initial number of mRNA copies of genetic makers transfected by co-culturing in a liquid medium with addition of Tween 20 is on the average 32% lower than when using modified heptamethyltrisiloxane
Журнал: Key Engineering Materials
Выпуск журнала: Т. 683
Номера страниц: 506-510
ISSN журнала: 10139826
Издатель: Trans Tech Publications Ltd.